Effects of cypermethrin on DNA breakage in the liver of Zebrafish (Aphanius sophiae) under different nutritional status

Cypermethrin is one of the most effective pesticides that gets into aquatic ecosystems. At the present study, effects of frequency of feeding on toxicity of cypermethrin were investigated in Zebrafish (Aphanius sophiae). The specimens were exposed to different treatments for 14 days, i.e. (1) the absence of poison and feeding every three days in the morning, (2) the absence of poison and feeding every day (morning and evening), (3) the presence of poison and feeding every three days in the morning and (4) the presence of poison and feeding every day in the morning and evening. Then the liver was sampled and its DNA was extracted. The DNA was run on a electrophoresis gel and the percentage of breakage was calculated using fuzzy logic contol method. The results showed a highly significant effect of feeding frequency and poison on DNA breakage.