Detection of potato virus Y (PVY) strains using RT-PCR in potato fields in Golestan province

Potato virus Y one of the most common and destructive viruses found in potatoes (Solanum tuberosum L.), and has a worldwide distribution. Generally, PVY have three strains including PVYN، PVYC، PVYO and two recombinant strains PVYNTN and PVYWilga. In order to determine the strains of the virus in the Golestan province samples were collected from potato fields. Samples were verified using an antiserum against PVY by indirect ELISA and posetive samples used in purvey of cDNA and RT-PCR test. Final PCR product was sequenced and comparison was made between these isolates and a number of other PVY strains available in GenBank. Three strains PVYN, PVYO and PVYWilga was reported first time in this province. In phylogenetic analysis showed that investigated strains were grouped into 4 groups. In this tree, Iranian isolate had most similarity with strains of Poland, Germany, Canada and Brazil. PVYN with 64. 03 percent distribution as the dominant strain has spread in potato fields in Golestan province.