Investigating the genetic variation in the collection germplasm Sistan grapevine cultivars using IRAP marker

To study the genetic diversity of Sistan grape cultivars, 33 grapes varieties (Vitis vinifera L) of Zahak Research Station were evaluated by 10 retrotransposons based primers from ‘Ty1-copia’ and ‘Ty3-gypsy’ families (IRAP). The observed polymorphism for any markers Vine1Fb, Gret1F.Rb, Gret1F.Ra and Gret1Rb were 54.5, 83.0, 85.7 and 85.7%, respectively. The results, revealed this probability that the Gret1 retrotransposon in evolution process of these plants has been more translocated and amplified the number of additional copies within the genome. Cluster analysis based on Jaccard's similarity coephicient and UPGMA algorithm was divided the population into two groups at 0.24 similarity coephicient; one group with ten sub-groups and one group with two varieties. The most number of effective allele and the most diversity were related to VineF.b primer with 0.6565 Shannon’s index and the least number of effective allele and the least diversity were related to Gret1R.a and VineF.R.b primers with 0.4226 Shannon’s index, respectively. The results of principal coordinate analysis revealed the results of cluster analysis and extracted six coordinates that explained 45.5% of the total variation of the studied population.