A Comparative Study of Effect of Types of Hibiscus SabdariffaExtracts and Selective Antibiotics on Clinical and Standard Strains of Infection Agents in Vitro

Nowadys, abundant research was done for replace of medicinal plants; plant antimicrobial components with structure of different from antibiotics cause destroy of bacteria that are clinically important. So, the present study was carried out with purpose of antimicrobial effect investigation of different types of Hibiscus Sabdariffaextracts on some clinical infection agent strain and comparison with common medical antibiotics in culture media condition. In this study, three types of ethanolic, methanolic and aqueous extracts were prepared by method of maceration. Microbiology evaluation of strains was done using four pour plate, Kirby Bauer method using disks, minimum inhibitory concentration of microbial growth (MIC) and minimum bactericidal concentration of bacteria (MBC) using Serial Dilution Method, onto six strains of infection agent. Turbidity of micro plate was determined by ELISA reading devices. The ethanolic extract of Hibiscus Sabdariffahas a diameter of 20.16 ± 0.50 mm in concentration of 40 mg/mL on Pseudomonas auerginisa. In the present study, the bacteria of staphylococcus aureus and Salmonella enteritidis were showed maximum and minimum sensitivity relation to aqueous extracts of Hibiscus Sabdariffa, respectively. Among of tested strains, Salmonella enteritidis has maximum MIC and MBC. 30 and 40 mg/mL Concentrations of Redcurrant have significant antimicrobial effect on bacteria. Antibacterial effect of extracts was decreased with decrease of extract concentration in disk. According to result, alcohol extracts of Hibiscus Sabdariffahave antimicrobial effect on growth of all of the strains exposed analyzes and antimicrobial effect of that was maximum on Gram-positive bacterum of Staphylococcus aureus.