Phytoremediation ability and oxidative enzymes activity of Persian clover (Trifolium resupinatum L.) in the presence of nickel

Phytoremediation is a relatively new method in agricultural science for removing soil contaminants to some extent through cultivating some plants with the least cost. This research was conducted to examine the role of nickel as a heavy metal in catalase and peroxidase activities and also to study uptake and transfer ability of nickel in the organs of Persian clover. Experiment was done as randomized complete blocks design with four treatments. Treatments were consumption of Ni (NO3)2 at four levels of 0, 100, 200, 300 ppm. The plants were treated for 10 days. The results of research showed that the increase in nickel concentration reduced LWCA and the fresh weights of shoot and root; however, it had no effect on the dry weight of shoots, RGR, and leaf area. Moreover, nickel concentration in aerial organ was higher than in roots. The results showed that catalase and peroxidase activity increased by increase of nickel in the nutrient solution. The results of this research indicated that protein content decreased as nickel increased. TF was greater than 1 but BCA and BCF were less than 1 and with increase in soil Ni so did ratios value. Therefore, Persian clover is not suggested as a suitable plant for phytoremediation.