The effect of diazinon exposure on hepatic tissue and enzymes in male frog Rana sridibunda

The purpose of this study was to examine the acute and sub-acute effects of diazinon on histopathological parameters of liver tissue and blood level of hepatic enzymes of male common frog (Rana ridibunda). Frogs were assigned to 9 groups (n=8, meaning 8 frogs in each group): control and treatment groups with 30, 60, 90 and 120 μg/L diazinon in water for one day or one week. Hepatic enzymes containing alanine transaminase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP) were measured using biochemical kit. All ALP values were significantly different between the control group and one-day and one-week diazinon exposed groups. Hepatocyte changes were observed only for diazinon concentrations greater than 60 μl after one week. The most important characteristics of the affected cells were hyperplasia and chromatin formation. Results show that sub-lethal concentrations of diazinon have an increasing effect on the blood level of hepatic enzymes, which probably indicated the detoxification response of liver on the basis of diazinon sub-lethal effect model.