Determination of Resistance to Klindamycine and Erythromycin of Staphylococcus aureus Clinical Isolates Obtained from Pathology Labratories in Sanandaj City

Staphylococcus aureus successfully colonizes humans, contaminates the hospital environment and has the genetic versatility for acquiring resistance to multiple antimicrobial agents. This study was conducted to determine the prevalence and genetic basis of erythromycin and clindamycin resistance in staphylococcus aureus isolates from Sanandajian patients.
One hundred and fifty clinical isolates of S. aureus were collected from Sanandaj Hospitals. Susceptibility to antibiotics (erythromycin and clindamycin) were determined by disk agar diffusion on Muller-Hinton agar as described by the Clinical and Laboratory Standards Institute (CLSI). The strains Staphylococcus aureus were screened by polymerase chain reaction (PCR) for the presence of five common erythromycin and
clindamycin genes resistance determinants, respectively, ermA, ermB, ermC, mphC, msrA.
Findings: Using the DAD (Disk Agar Diffusion) method , the researchers found that 56/4% of the Staphylococcus aureus isolates were resistant to erythromycin and 56/8% to clindamycin. Furthermore, the ermA gene was found in 79 isolates, ermB in 36 isolates, ermC in 62 isolates and mphC, msrA were detected in 16 and 29 isolates, respectively, by PCR technique.
Discussion & This study indicates that resistance to erythromycin is mainly mediated by ermA and ermC genes in S. aureus in sanandaj city.