An investigation on phylogenetic classification of Helicoverpa armigera Nucleopolyhedrovirus (HaNPV), isolated from Tobacco fields of north of Iran, by RAPD markers

For screening Helicoverpa armigera Nucleopolyhedrovirus (HaNPV) isolated from Tobacco fileds of North of Iran, Mazandaran & Golestan Provinces, among 10 RAPD (10bp) primers of Operon that randomly selected, two RAPD primers (OPM-03 and OPM-16) have highest polymorphism and selected. Phylogenetic analysis was done using PyElph software and UPGMA method. Then, phylogenetic tree and genetic distances table were drawn. The cluster analysis divides the 16 HaNPV isolates in to five distinct groups. Such screening of HaNPV isolates is useful for improving biological control measures against tobacco budworm and cotton bollworm on different crops.