Evaluation of CD4+CD25+FOXP3+ Regulatory T cells in Tumor Environment of Invasive Intraductal Breast Carcinoma and Control group

Aim and it seems that population of regulatory T cells increases in many malignancies leading to suppression of effector immune responses and thereby to progression of the disease. We aimed to assess infiltration of the regulatory T cells into tumor environment in women with breast carcinoma.
in a control-case study, 9 malignant tumor tissues from invasive intraductal breast carcinoma (case) and 8 benign breast tissues from healthy women (control) were mechanically and enzymatically processed. The prepared cell suspension was then subjected to staining of cell surface markers CD4 and CD25, and the extracellular marker FOXP3, and were finally analyzed by flow cytometry.
population of regulatory T cells in malignant tumor tissues showed significant increase compared to the control (P increased regulatory T cells population in invasive intraductal breast tumor environment may be the major cause of immunosuppression contributing to the tumor progression. However, further studies with larger sample size are suggested to clarify this hypothesis.