Phytochemical, Antioxidant, and Antibacterial Properties of Medical Plant Salvia sharifii Rech. f. & Esfand

Salvia sharifii as medicinal plants has many therapeutic properties including; anti- microbial, anti- flatulence and anti- rheumatism. The goal of this study was to identify the active ingredients, antibacterial and antioxidant of aerial parts of Salvia sharifii collected from East of Iran.
The active component of the methanolic extract were separated and identified by gas chromatography (GC) and gas chromatography- mass spectrometry (GC-MS) methods. The essence of the aerial parts was extracted by Clevenger apparatus. The effect of essence against Staphylococcus aureus and Escherichia coli was tested by the micro dilution method. The total phenolic contents of the extracts were determined by Folin-Ciocalteu method and the antioxidant activity was evaluated using DPPH (2, 2 -diphenyl 1-1 picrylhydrazyl) assay.
The result of this study revealed that Bornyl acetate (20.85%), - Caryophyllene (15.79%) and Camphene (15.63%) were the most prevalent components of the methanolic extract. The minimum inhibitory concentration of the essential oils against Staphylococcus aureus and Escherichia coli were 3/75mg/ml and 7/5mg/ml respectively. Also, The amount of phenolic component of the methanolic extract was 92 µg/mg and the antioxidant activity of methanolic extract and essence were 32.1 and 58.2 µg/m respectively.
present Bornyl acetate in Salvia sharifii, the major compound in plant extract plays an important role in antimicrobial activity. Methanolic extract of Salvia sharifii has strong antioxidant activity.