Antibiotic resistance in Staphylococcus aureus isolated from clinical samples by disk diffusion and PCR methods

Author(s):

Kavoosinezhad F. , Fattahi E. * , Moori Bakhtiari N

Message:
Abstract:
Background And Objective
Resistance of Staphylococcus aureus to antibiotics is one of the major global health problems in human societies. Thus, evaluation of pattern of antibiotic resistance in its different strains is very important. This study was carried out to evaluate the antibiotic resistance in Staphylococcus aureus isolated from clinical samples by disk diffusion and PCR methods.
Methods
In this laboratory- descriptive study, 50 isolates of Staphylococcus aureus to be identified from clinical specimens. Methicillin resistance was examined using PCR and antibiotic susceptibility of isolates was tested by disk diffusion method.
Results
50 isolates were resistant to methicillin, ampicillin and penicillin. The resistance of isolates to erythromycin, Gentamicin, Clindamycin and Ciprofloxacin were 48%, 34%, 34%, 34%, respectively. The PCR method showed that 98% of Methicillin Resistance of Staphylococcus aureus isolates carried the methicillin resistant gene.
Conclusion
This study indicated that 98% isolates harbor mecA genes and more resistant to methicillin related mecA genes.
Keywords:

Staphylococcus Aureus , Antibiotic Resistance , Disk diffusion method , PCR

Language:
Persian
Published:
Journal of Gorgan University of Medical Sciences, Volume:18 Issue: 2, 2016
Pages:
115 to 119
https://www.magiran.com/p1545531  
سامانه نویسندگان
  • Fattahi، Esmail
    Corresponding Author (2)
    Fattahi, Esmail
    Associate Professor Department of biology, 437348 Branch, Islamic Azad University, آمل, Iran
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