Frequency of genes encoding erythromycin ribosomal methylases among Staphylococcus aureus clinical isolates with different D-phenotypes in Tehran, Iran
Author(s):
Abstract:
Background And Objectives
Macrolide, lincosamide and streptogramin type B (MLSB) antibiotics are important in the treatment of Staphylococcus aureus infections and existence of isolates with ability to resist against MLSB antibiotics is worrisome.Materials And Methods
In this cross sectional study, 101 S. aureus isolates were collected from patients of five selected hospitals in Tehran over a period of five months. Disk diffusion tests and differentiation between constitutive and inducible resistances were carried out by D-test. The presence of mecA, msrA, ermA and ermC genes were detected using PCR or multiplex PCR.Results
Out of 101 S. aureus isolates, 58 (57.4%) were methicillin resistant and 57 (56.4%) expressed resistance to erythromycin. The prevalence of constitutive MLSB (cMLSB), inducible MLSB (iMLSB) and MS (Negative) phenotype in all erythromycin resistant isolates were 71.9, 26.3 and 1.7%, respectively. Out of all the erythromycin resistant isolates, 57.8% harbored both ermA and ermC genes which possessed constitutive resistance. 8.7% of the isolates contained ermA gene alone which possessed inducible resistance with D phenotype and 5.2% of isolates just contained ermC gene which had inducible resistance with D phenotype. msrA gene was detected in 3.5% of the erythromycin resistant S. aureus isolates with constitutive resistance. None of the genes were detected among MS phenotypes.Conclusion
In this study, most of S. aureus isolates carried both ermA and ermC genes and there was a significant relationship (P value ≤ 0.05) between different resistance phenotypes and erm genes.Language:
English
Published:
Iranian Journal of Microbiology, Volume:8 Issue: 3, Jun 2016
Pages:
161 to 167
magiran.com/p1565701
دانلود و مطالعه متن این مقاله با یکی از روشهای زیر امکان پذیر است:
اشتراک شخصی
با عضویت و پرداخت آنلاین حق اشتراک یکساله به مبلغ 1,390,000ريال میتوانید 70 عنوان مطلب دانلود کنید!
اشتراک سازمانی
به کتابخانه دانشگاه یا محل کار خود پیشنهاد کنید تا اشتراک سازمانی این پایگاه را برای دسترسی نامحدود همه کاربران به متن مطالب تهیه نمایند!
توجه!
- حق عضویت دریافتی صرف حمایت از نشریات عضو و نگهداری، تکمیل و توسعه مگیران میشود.
- پرداخت حق اشتراک و دانلود مقالات اجازه بازنشر آن در سایر رسانههای چاپی و دیجیتال را به کاربر نمیدهد.
In order to view content subscription is required
Personal subscription
Subscribe magiran.com for 70 € euros via PayPal and download 70 articles during a year.
Organization subscription
Please contact us to subscribe your university or library for unlimited access!