A Comparative Study of Activity and Stability of the Free and the Immobilized Endoglucanase from Alicyclobacillus Acidocaldarius
Author(s):
Abstract:
AaCel9A [β-1,4-endoglucanase, (E.C:3.2.1.4)], was immobilized onto glutaraldehyde activated chitosan macrosphere by covalent attachment. The properties of the immobilized AaCel9A were investigated by determining the optimum pH and optimum temperature for activity, thermal stability, and kinetic parameters. The immobilization process shifted the enzymes optimum temperature from 65 °C for the free enzyme towards a wider temperature range from 60-80 °C by the immobilized enzyme. The optimum pH of immobilized AaCel9A shifted to basic pH (pH 8) relative to free AaCel9A (pH 6.5). The immobilization on chitosan macrosphere enhanced half-life of AaCel9A enzyme. After 60 min, the immobilized and the free enzyme retained 75% and 40% their activity at 65 °C, respectively. The immobilized enzyme showed higher thermal stability than the free form. Km value of immobilized AaCel9A (17.05 mg ml-1) was higher than free AaCel9A (7.75 mg ml-1). Also, CMC hydrolysis by immobilized and free AaCel9A in the presence of SDS detergent was investigated. The results showed that the immobilized enzyme maintained its activity more than the free form in different concentrations of SDS.
Keywords:
Language:
English
Published:
Biomacromolecular Journal, Volume:1 Issue: 2, Autumn 2015
Pages:
167 to 176
magiran.com/p1576990
دانلود و مطالعه متن این مقاله با یکی از روشهای زیر امکان پذیر است:
اشتراک شخصی
با عضویت و پرداخت آنلاین حق اشتراک یکساله به مبلغ 1,390,000ريال میتوانید 70 عنوان مطلب دانلود کنید!
اشتراک سازمانی
به کتابخانه دانشگاه یا محل کار خود پیشنهاد کنید تا اشتراک سازمانی این پایگاه را برای دسترسی نامحدود همه کاربران به متن مطالب تهیه نمایند!
توجه!
- حق عضویت دریافتی صرف حمایت از نشریات عضو و نگهداری، تکمیل و توسعه مگیران میشود.
- پرداخت حق اشتراک و دانلود مقالات اجازه بازنشر آن در سایر رسانههای چاپی و دیجیتال را به کاربر نمیدهد.
In order to view content subscription is required
Personal subscription
Subscribe magiran.com for 70 € euros via PayPal and download 70 articles during a year.
Organization subscription
Please contact us to subscribe your university or library for unlimited access!