Evaluation of Immobilized Bacteriorhodopsin's Function by Laser Irridiation
Bacteriorhodopsin (BR) is a retinal protein that is a light-driven proton pump and has an important role in photosynthesis in archaebacterium Halobacterium salinarum. The BR molecule absorbs light and photochemical changes occur in it, and different intermediates will be produced in its photochemical cycle that some of them like P and Q intermediates have a long half-life. There have been many efforts to immobilize BR for constructing data storage devices. In this study the BR suspension-contained film has been immobilized on a polycarbonate and the effects of green and red lasers on it (in different times and temperatures) have been investigated by UV spectrophotometer method, The modified surfaces were characterized by ATR-FTIR and AFM techniques. The results show that with used condition for immobilization of BR on polycarbonate, the 0 and 1 bite that relate to BR and P, Q intermediate, are formed. The red and green lasers convert BR to O and, P or Q intermediates respectively that could be used instead of 0 and 1 bites in popular compact disks.
Biomacromolecular Journal, Volume:1 Issue: 2, Autumn 2015
196 to 203
دانلود و مطالعه متن این مقاله با یکی از روشهای زیر امکان پذیر است:
اشتراک شخصی
با عضویت و پرداخت آنلاین حق اشتراک یک‌ساله به مبلغ 990,000ريال می‌توانید 70 عنوان مطلب دانلود کنید!
اشتراک سازمانی
به کتابخانه دانشگاه یا محل کار خود پیشنهاد کنید تا اشتراک سازمانی این پایگاه را برای دسترسی نامحدود همه کاربران به متن مطالب تهیه نمایند!
  • حق عضویت دریافتی صرف حمایت از نشریات عضو و نگهداری، تکمیل و توسعه مگیران می‌شود.
  • پرداخت حق اشتراک و دانلود مقالات اجازه بازنشر آن در سایر رسانه‌های چاپی و دیجیتال را به کاربر نمی‌دهد.
In order to view content subscription is required

Personal subscription
Subscribe magiran.com for 50 € euros via PayPal and download 70 articles during a year.
Organization subscription
Please contact us to subscribe your university or library for unlimited access!