Study of camel and goat Sarcocystis by electron microscopic and PCR- RFLP

Sarcocystis is one of the largest genera of the protozoan phylum apicomplexa, order sporozoa. It is one of the most prevalent parasites in domestic and wild animals, and is a worldwide distributed parasite. Certain species also infect humans, therefore it has economic and public health importance as a zoonosis. Diagnosis of Sarcocystis in camels and goats with Electron microscopy and PCR- RFLP methods and discriminating of different species of Sarcocystis in different hosts was carried out. 166 small pieces of tissues were collected from freshly slaughtered camels and goats by squash and trypsin digestion techniques were applied for the detection of the cysts. The infected organs fixed in glutaraldehyd and frozen at -20 until use for ultrastracturtural and molecular study, respectively. In this study no macrocyst were found in the samples of camels. In camels only microcysts were observed. The cysts in the goats may be probably S.moulei species. But to verify this statement needs more investigation. The results of this study will be helpful in discriminating of different species of Sarcocystis in different hosts. Based on ultrastructural cyst wall and molecular information of DNA sequences in goat, fat and thin macrocysts represent one species.