The Optimization of Callus Induction and Regeneration in Medicinal Lemon Verbena (Lippia citriodora) Plant

This study were studied for evaluation of the effect of growth regulators type and interaction on callus induction and plant regeneration of Lippia citriodora, in Murashige and Skoog (MS) medium. The experiment of callus induction with leaf and stem explants, were arranged as factorial experiment based on completely randomized design (CRD). Also, experiment of regeneration performed in completely randomized design. For evaluate of callus induction, combination of 2,4-D with BAP and with NAA were applied. In regeneration study, combination of BAP with Kin and with NAA were used. The effects of these plant growth regulators on callus induction of leaf explant showed that treatment of 0 Mg-1l NAA and 2.0 Mg-1l 2,4-D showed the highest (97%) rate of callus induction. The highest percentage of callus formation were observed in stem explant at 0 Mg-1l NAA and 2.5 Mg-1l 2,4-D (97%) treatment. Callus induction on MS medium without plant growth regulator was not observed for leaf and stem explants. The highest percentage of shoot production (40%) in 1.0 Mg-1l BAP 0.05 Mg-1l Kin and highest percentage of root production (40%) with combined plant growth regulator of 1.0 Mg-1l BAP 0.05 Mg-1l NAA were observed. According to these results, in vitro culture of the Lippia citriodora can be used to fast and easy propagation of this plant.