Applying the Bioinformatics Methods to Design and Evaluate the SapM-M13 pIX Fusion Protein and its Theoretical Role in Phage ELISA System

Phage ELISA is a common method to confirm binding of obtained phages from phage display technique to related antigens. Enzyme-conjugated antibody directed against the major capsid protein (pVIII) or enzyme-conjugated secondary antibody against the primary antibody is used as a detection system in phage ELISA. We have suggested to express an acid phosphatase (SapM) enzyme on M13 pIX minor coat protein directly, and evaluated this hypothesis using insilico techniques. We believe that decreasing the antibody layers in phage ELISA will significantly increase the reliability and reproducibility of the test and reduce its time.