The effect of gamma irradiation on the biological property of hydroalcoholic extract and essential oils derived from Chelidonium majus L

In this study, the composition and biological properties of Chelidonium majus L. hydroalcoholic extract and the essential oils and also the effect of gamma irradiation on their biological properties were investigated. For this purpose, Chelidonium majus were collected through the establishment of plots from sisangan-nowshahr. Plant samples were dried at room temperature and away from sunlight for one week. Parts of samples were transferred to the Radiation Application Research Institute, irradiated at 10 and 25 kGy. Then, the essential oils extracted using Clevenger apparatus and its compounds were analyzed by GC / MS. Then, the hydroalcoholic extraction were made following estimation of flavonoid rate with standard solutions. Then, the antioxidant activities of the samples were determined by beta-carotene and DPPH tests. Based on the results of the analysis of GC / MS, 22 , 18 and 26 compounds were identified in control, 10 and 25 kGy irradiated samples, respectively. The highest flavonoid content belongs to 25 kGy irradiated samples and the lowest belongs to control samples, but these changes were not significant. Also, control and irradiated essential oils and extracts, significantly indicating strong antioxidant activities in beta-carotene and DPPH tests in compared with references. According to the results, oil and extracts derived from Chelidonium majus had certain compounds along with significant antioxidant activities. Gamma radiation had no significant effects on the compounds and antioxidant activities of plant sample.