Study of Relationship between Polymorphism in Estrogen Response Element (ERE) in Promoter Region of C3 Gene and Spontaneous Recurrent Abortion
BackgroundMiscarriage is one of the most common pregnancy complications for which various causes have been defined, such as genetic factors, infectious, metabolic, endocrine systemmal function and immune system undesired responses. The early development of embryo occurs in oviduct and uterine tube from which some factors such as growth factors, glyco-proteins and factors those stimulate development of embryo are secreted. The ETF3 embryotrophic factor which is a complex of C3 complements and its derivatives i.e., iC3b, enhances the development of trophectodermas a consequence of which expression of relevant genes are affected embryo. There are various response elements in C3 gene promoter region such as, estrogen response regions (ERE). Steroids such as estrogen and progesterone are secreted in early steps of embryonic period along with C3 secretion and cause increase in C3 expression through interaction with regulatory elements in promoter region of this gene. In this study the polymorphism in ERE regions of C3 gene promoter was investigated in women suffering from recurrent miscarriage.
Materials And MethodsIn this study, assuming that polymorphism in ERE regions is correlated with recurrent miscarriage during early months of pregnancy, 40 blood samples were collected from female patients admitted to an Infertility Clinic, Isfahan, Iran. DNA was extracted, amplification of regions harboring ERE with a pair of specific primer was done using Polymerase Chain Reaction-Single Strand Chain Polymorphism (PCR-SSCP) for studying possible polymorphisms in this region.
ConclusionThe results indicated a specific symptomless infertility among the women, however there was no correlation between the ERE polymorphism and symptoms in control and cases.
Journal of Molecular and Biochemical Diagnosis, Volume:1 Issue:2, 2015
135 - 142
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