Differential Expression of MiR-18b in PBMC from T1D Patients in Isfahan population
Author(s):
Abstract:
Background
Type 1 diabetes (T1D) is caused by cell-mediated autoimmune attack on pancreatic beta-cells. Previous studies highlight the role of microRNAs (miRNAs) in the pathogenesis of T1D. MiRNAs are small non-coding RNAs involved in the regulation of gene expression post-transcriptionally. In this work, miR-18b was chosen and the differential expression of it was measured between T1D patients and healthy controls from Isfahan population.Materials And Methods
MiR-18b was selected using Bioinformatics studies by miRWalk software. 22 T1D patients and 18 healthy controls from Isfahan population were enrolled in this study. Total RNA of the peripheral blood mononuclear cells (PBMCs) samples were extracted. After cDNA synthesis, the expression profile of miR-18b quantified by means of qPCR method in patients and controls. Finally the results were statistically analyzed.Results
In this study despite our hypothesis, the expression levels of miR-18b didnt show any significant difference between T1D patients and healthy controls (p value: 0.145).Conclusion
Due to the results of our experimental analysis, it seems that miR-18b doesnt have any association with T1D disease in Isfahan population.Language:
English
Published:
Research in Molecular Medicine, Volume:4 Issue: 4, Nov 2016
Pages:
45 to 50
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