The effect of Origanum Vulgare extract as natural antioxidant on quality cryopreserved ram sperm

Oxidative stress during freeze-thawing process causes reduction in motility, viability, membrane functions and finally sperm fertility.
the aim of this study was to determine the effect of Origanum Vulgare extract as natural antioxidant on quality cryopreserved ram sperm.
In this study semen was collected from five mature ram twice a week using an artificial vagina and the ejaculates were pooled in order to eliminate the individual effect of rams. Different levels of Origanum Vulgare extracts (0, 100,150 and 250 µL/mL diluent solution) were added to diluent based tris-egg yolk. After cooling, filling and sealing of the samples, they were frozen and with nitrogen vapor and immersed in liquid nitrogen and were stored until evaluation time.
After thawing, results showed that addition of 150 µL/mL of extract increased progressive motility compared to the control groups (P The obtained data showed that inclusion of Origanum Vulgare extract in semen freezing extender improved post thawed motility of ram sperm.