The Survey of Antibacterial Effect of Rossadamascena Extract Polar، Semi-Polar And Non-Polar Fractions on Staphylococcus Aurous، Escherichia Coliand Pseudomonas Aeruginosa Bacteria

Although antibiotics are used in the treatment of infectious diseases¡ there are many problems such as adverse drug reactions and resistance to antibiotics. Plants can be considered as a substitute for synthetic drugs.Rossadamascena is one of medicinal plants which has a long history in traditional medicine And is used in the treatment of many diseases. In this comparative studies Antibacterial effect of Rossadamascena extract polar¡ semi-polar and non-polar fractions on Staphylococcus aurous¡ E.coli and Pseudomonas aeruginosa bacteria. Rossadamascena plant was used in this study.Initially extracts of methanol¡ chloroform and ether of Rossadamascena was prepared and the effect of different concentrations of the extracts were evaluated.All experiments were performed with the release of the wells and determine the MIC and MBC on standard strains of Staphylococcus aureus¡Escherichiacoli¡ and Pseudomonas aeruginosa. Chloroform extract of Rossadamascena plant at a concentration of 12.5 micrograms per milliliterinhibits the growth of the Gram-positive bacteria Staphylococcus aureus by microplate.Whereas¡ higher concentrations are needed to effecting on gram-negative bacteria Escherichiacoli and Pseudomonas aeruginosa. Chloroform extract of Rossadamascena plant have most inhibitory and killing effects on Gram-positive Staphylococcus aureus; and the methanol extract of this plant in concentration of 25 micrograms per milliliter and have more killing and inhibitory effects on Gram-positive bacteria Staphylococcus aureus.