Application of Magnetic Polymer Particles Modified with β- Cyclodextrin for Adsorption of Bovine Serum Albumin

Magnetic polymer particles which were modified by vinyl groups and subjected to polymerization by vinyl β-Cyclodextrin derivative, has been used as adsorbent for sorption and release of bovine serum albumin and the equilibrium and kinetics of the adsorption process were studied. The absorbability and releasing of this protein through the new polymer has been measured by ultraviolet-visible spectroscopy. Effects of various parameters such as pH, contact time and capacity for adsorption of bovine serum albumin have been studied in vitro and the optimum magnitudes were 4, 37.94 mg/g and 5 minutes respectively. The experimental results show that the adsorption of Bovine serum albumin on magnetic particles was affected greatly by the pH andthe adsorption equilibrium isotherm was fitted well by the Freundlich model. Bovine serum albumin was released from magnetic particles in simulated intestinal fluids. It was released up to about 7 h at high speed and then it released slowly up to 30 h.