Localization and Activity of Mouse Endometrial Alkaline Phosphatase after Hyperstimulation and Progesterone Injection at the Implantation Time

The activity of mouse endometrial alkaline phosphatase after hyperstimulation and progesterone injection at the implantation time Alkaline phosphatase (ALP) of endometrium may play a critical function in the development and implantation of embryo. The aim of this study was to determine the localization of endometrial ALP activity after hyperstimulation and progesterone injection. Thirty adult female NMRI mice were hyperstimulated using human menopasual gonadotropic hormone and human chorionic gonadotropic hormones (hCG). Then, daily injections of progesterone (1 mg/mouse) were performed in one hyperstimulated group. Animals were sacrificed by cervical dislocation 4.5 day after hCG injection. Tissues were obtained from 1/3 middle part of uterine horns and used for enzyme histochemistry and morphological studies. The samples were cryosectioned at -30°C and the enzyme histochemistry was carried out by azo-coupling technique using alpha naphtol phosphate as substrate. In the control groups, the ALP activity was localized on the apical and basal border. Cytoplasm of glandular and surface epithelium was stronger than the other hyperstimulated groups on the day four of pseudoprgnancy. Our results showed that the ALP activity was decreased after ovarian hyperstimulation and progesterone injection and the defect of implantation of embryo after hyperstimulation and embryo transfer in animals may be due to the reduction of enzyme activity.