Microbio-ecology and hydro-geochemistry of saline sulfur springs of Ghale-Madreseh, Khuzestan, Iran

Ghale-madreseh is the first point that the saline and sulfurous streams flow into Tembi River, one of the well-known saline rivers in Khuzestan province, Iran. This river is one of the main sources of increasing Karun River’s salinity, which is the largest river in Iran in terms of discharge. There are three saline and sulfurous springs (Shour-1, Shour-2m and Namak Springs) as well as a drinkable one (Shireen spring) in Ghale-madreseh region. Normally, most probable number counting of sulfate reducing, sulfur oxidizing and nitrate reducing bacteria showed that there are different patterns of microbial populations in the springs of Ghale-madreseh region. The observed differences are highly attributed to the hydro-geochemical properties of the springs. It is assumed that the groundwater which streams in the Gachsaran formation receives considerable amounts of SO42- (0.09-0.1 M), Na (1.654-3.604 M), and Cl- (1-548-3.775 M) by halite and gypsum dissolution, resulting in the saline and sulfurous springs on the Gachsaran formation. Also, due to the low depth of local oil reservoirs, activity of sulfate reducing bacteria in the close vicinity of oil reservoirs and groundwater streams is highly probable. Hence, the microbial sulfate reduction may be responsible for the production of H2S, probably playing a role in the souring of local oil reservoirs. Besides, the groundwater that reaches the Bakhtiary formation shows different characteristics as detected in Shireen spring.