Isolation, characterization and standard storage of human mesenchymal stem cell derived from adipose and dental pulp tissue

Due to extensive use of cell cultures in biomedical investigations and researches, there has been a great need to identify, authenticize and accurate quality control of cells. Stem cells are characterized by their self-renewal and differentiation potential. Mesenchymal stem cells are found in various tissues. These cells are vastly used in cell therapy and regenerative medicine and many other cell related studies.
After separation of cells from adipose and dental pulp tissues and confirmation of quality controls, authentication of mesenchymal stem cells were determined by monitoring growth, morphology, expression of specific molecular markers, and the ability to differentiate into adipocyte and osteoblast.
In this study, 6 mesenchymal stem cell lines were established from adipose and dental pulp tissues. These cells which were positive for CD90, CD105 and CD29 and negative for CD34 and CD45 expression were isolated, authenticated and stored. Moreover, these cells showed no contamination including bacterial, viral and mycoplasma and are able to differentiate into adipocyte and osteoblast in vitro.
The outcome of this project would be introducing authenticated mesenchymal stem cells with related identity documents which facilitate and accelerate the biomedical research in the field of human mesenchymal cells along with a substantial reduction of costs and time.