Green Synthesis and Antibacterial Effect of Silver Nanoparticles by Using Extract of Citrus aurantium

There are many different methods for the production of silver nanoparticles (Ag-NPs) because of these nanoparticles can be used for multiple applications. Recently, using of plants in the synthesis of nanoparticles due to a cost effective and eco-friendly approach has been extensively noticed. In this study, the biosynthesis of silver nanoparticles by using extract of Citrus aurantium and its antimicrobial properties has been reported.
in this study, the extract of Citrus aurantium as the reducing agent is used for the biosynthesis of silver nanoparticles. Reduction of Ag ion was obtained after 24 h by using extract of Citrus aurantium in presence of 1 mM silver nitrate (AgNo3) solution. The size, structural, optical and morphological properties of nanoparticles have been analyzed by facility of: X-ray Diffraction, Field Emission Scanning (Transmission) Electron Microscope.
UV-Vis spectrum exhibit an absorption band at around 400-450 nm suggestion the formation of biological Ag nanoparticles. Size and morphological properties of nanoparticles was performed by TEM which show that particles have spherical shape with diameter of about 5-40 nm. Silver nanoparticles presented the antimicrobial activity against Gram positive and Gram negative bacteria.
The extract of Citrus aurantium has ability of Ag ion to reduce to Ag nanoparticles. Also, Ag nanoparticles produced by Green Synthesis had proper antibacterial activity.