The study of soil chitinolytic bacterial strains and their use against fungal pathogen Alternaria alternata in vitro

In sustainable agriculture, biological control is an important alternative to control various plant diseases, in particular, pathogenic fungus that causes the most economic damage to agricultural products in the world. Each soil species bacterium has unique specified features and abilities. Alternaria sp has specific herbal poisons that lead to diseases in them. Biological control of fungal diseases in agricultural products across the world has been done in recent years using fungi and soil antagonist bacteria. Some bacterial isolates that produce chitinase enzymes fight with fungal pathogens.
To achieve an antagonist chitinolytic bacteria against Alternaria alternata fungal, screening soil species bacterium that produce chitinase of some urban and agriculture soil samples of Qazvin were carried out.
In this study, five different strain of chitinolytic bacteria were found, two strains of Pseudomonas nitroreducens in vitro and one strain (AHH4), they had the ability to destroying fungal pathogen Alternaria sp in the farm. as the presence of high concentrations (0/05 OD600 and above) of the bacteria, A. alternata fungus growth stopped completely.
Discussion and However, one of them, P. nitroreducens (AHH4) could control the fungusat in vitro and invivo and inhibit the growth of fungus. So P. nitroreducens (AHH4) chitinolytic bacteria has efficiently effect on inhibit A. alternata fungal growth. As the presence of high concentrations (0/05 OD600 and above) of the bacteria A. alternata fungus growth stopped completely. The isolate AHH4 was submitted to NCBI/DDBJ/EMBL as KR905055 accession number.