Comparison of the effect of infant fecal microbiota on the cell viability of colon cancer cells and Unrestricted Somatic Stem Cells

The microbiota of breast-fed infants are mainly consists of Bifidobacterium and Lactobacillus. Normal vaginal delivery has an important role in the colonization of beneficial bacteria and proper development of the immune system and gastrointestinal tract in comparison with infants born through cesarean section. In this study we examined the effect of microbiota of a 4-month-old breastfeeding infant born with normal vaginal delivery, without history of taking antibiotics and gastrointestinal disorders on the cell viability of two types of cells with different growth features including colon cancer cells (Caco-2) and Unrestricted Somatic Stem Cells (USSCs). After fecal sampling, the microbiota was extracted by ethyl acetate. After removing the solvent, the result of extraction was solved in a few volume of DMSO. The effects at concentrations of 2.5 and 5 µg/ ml on the following cells were determined after 72 hours of incubation using MTT assay. The results showed that the cell viability of USSCs was significantly increased as the concentration elevated. While at the same conditions, the viability of Caco-2 cells was decreased at the higher concentration significantly.