Antibacterial Effect of Tragopogon graminifolius DC Hydroalcoholic Extracts on Acinetobacter baumannii(In vitro study)

Acinetobacter baumannii opportunistic pathogen causing nosocomial infection, many problems in success of treatment failure and mortality in patients because of resistance to antibiotics has created. Tragopogon graminifolius DC plant in traditional medicine in Iran and around of the world by having multiple health effects always have been used. The aim of this study was to invitro investigating of antibacterial effect of Tragopogon graminifolius DC hydroalcoholic extracts on Acinetobacter baumannii.
Material and In this study, plant Tragopogon graminifolius DC collected in the spring season and after confirming it was dried in the shade and away from direct sunlight. Extraction was performed by Maceration method and alcoholic and aqueous extracts obtained with concentrations of 6.25, 12.5, 25, 50 and 100 mg per ml prepared to do the work were stored at 4°C. Standard strains of Acinetobacter baumannii at these concentrations with disc diffusion method was determined susceptibility. inhibition zone diameter was measure and recorded.
The aqueous extract Tragopogon graminifolius DC in the highest concentration (10 mg per ml) caused an inhibition zone with a diameter of 2 mm, but alcoholic extract did not cause any inhibition.
Due to very weak growth inhibitory effect of aqueous extract and ineffective alcoholic extract it can be said, plant Tragopogon graminifolius DC has no antibacterial effect in Acinetobacter baumannii.