The Effect of Pomegranate Extract on Producing Type II Collagen in Differentiation of Adipose-Derived Stem Cells into Chondrocytes

Author(s):

Mehri Katani , Behzad Zolfaghari , Mitra Soleimani , Ali Valiani , Batool Hashemibeni

Article Type:
Research/Original Article (دارای رتبه معتبر)
Abstract:
Background
Cartilage tissue is avascular and has no repairing capacity. For cartilage tissue engineering, stem cells and growth factors are used. In this study, the effect of pomegranate as inducer for chondrogenesis of adipose-derived stem cells (ADSCs) was evaluated.
Methods
Human adipose-derived stem cells in third passage seeded in fibrin were cultured in chondrogenic medium with pomegranate for 2 weeks. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT assay) and western blot technique were applied for evaluation of differentiated cells.
Findings: Adipose-derived stem cells differentiated into chondrocytes; and type II collagen production by differentiated cells was proved.
Conclusion
Pomegranate extract is an appropriate inducer for production of type II collagen in adipose-derived stem cells.
Keywords:

Chondrogenesis , Stem cells , Pomegranate , Type II collagen

Language:
Persian
Published:
Journal Of Isfahan Medical School, Volume:35 Issue: 453, 2017
Pages:
1540 to 1545
magiran.com/p1779351  
دانلود و مطالعه متن این مقاله با یکی از روشهای زیر امکان پذیر است:
اشتراک شخصی
با عضویت و پرداخت آنلاین حق اشتراک یک‌ساله به مبلغ 1,390,000ريال می‌توانید 70 عنوان مطلب دانلود کنید!
اشتراک سازمانی
به کتابخانه دانشگاه یا محل کار خود پیشنهاد کنید تا اشتراک سازمانی این پایگاه را برای دسترسی نامحدود همه کاربران به متن مطالب تهیه نمایند!
اطلاعات بیشتر
توجه!
  • حق عضویت دریافتی صرف حمایت از نشریات عضو و نگهداری، تکمیل و توسعه مگیران می‌شود.
  • پرداخت حق اشتراک و دانلود مقالات اجازه بازنشر آن در سایر رسانه‌های چاپی و دیجیتال را به کاربر نمی‌دهد.
In order to view content subscription is required

Personal subscription
Subscribe magiran.com for 70 € euros via PayPal and download 70 articles during a year.
Organization subscription
Please contact us to subscribe your university or library for unlimited access!
More information