Analysis of Ala234Thr Polymorphism SEPP1 gene in Women with Breast Cancer in Guilan Province

Selenoprotein P (Sepp1) is the major selenoprotein in plasma and contains over 50% of the total plasma. The direct and indirect effects of Sepp1 on anti-oxidant defense can be responsible for its role in cancer risk. One of the common polymorphism of SEPP1gene is the Ala234Thr single nucleotide polymorphism (SNP) in coding region. In this research we studied the association between SEPP1 Ala234Thr (rs3877899) polymorphism with the risk of breast cancer.
In this case-control study, 162 patients with breast cancer were compared with 227 healthy subjects. Genomic DNA was extracted from peripheral blood leukocytes. Determination of DNA genotyping in SEPP1 Ala234Thr (rs3877899) polymorphism was performed by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) by using MwoI enzyme. Statistical analysis was performed by using the MedCalc program and χ2-test.
The genotype frequencies were significantly different between the cases and controls. AA homozygosity subjects were more at risk for breast cancer than others (OR=3.23; 95%CI, 1.79-5.84; p=0.001).
Based on the results of this study, The Ala234Thr polymorphism of the SEPP1 gene may be associated with the potential for breast cancer in the female population of northern Iranian women. However, studiesneed to be conducted in larger populations to confirm these results.