Study of CFL1 Gene Expression in the Intestinal Samples of Gastric Adenocarcinoma in East Azarbaijan Population

Gastric cancer is considered as the fourth most common cancer and the second most common cause of cancer-related death around the world. Northern areas of Iran from NorthWest to NorthEast have high risk of this cancer. Genetic mutations and changing in expression level are the most effective factors in carcinogenesis and cancer progression. CFL1 is one of the genes that is affected by changes in the level of expression. CFL1 is isoform of the ADF/cofilin protein familyThe protein encoded by this gene can polymerize and depolymerize F-actin and G-actin in a pH-dependent manner. In this research, we studied expression of this gene in intestinal samples of gastric adenocarcinoma.
Material and In this study, mRNA in intestinal type of gastric adenocarcinoma of 26 biopsy samples was extracted, then after cDNA synthesis, real-time polymerase chain reaction was performed to measure gene expression.
The rate of CFL1 gene expression in tumor samples significantly increased compared with healthy tumor margins (p This gene can be used as a biomarker in gastric cancer. Overall, the results of this study showed that the expression of CFL1 has significant association with the nature of cancer tumors. This implies that CFL1 can have high potential as novel targets for gene therapies in gastric cancer.