Hyperuricemia Induces Wnt5a/Ror2 Gene Expression, Epithelial- Mesenchymal Transition, and Kidney Tubular Injury in Mice
Author(s):
Article Type:
Research/Original Article (دارای رتبه معتبر)
Abstract:
Background
Hyperuricemia contributes to kidney injury, characterized by tubular injury with epithelialmesenchymal transition (EMT). Wnt5a/Ror2 signaling drives EMT in many kidney pathologies. This study sought to evaluate the involvement of Wnt5a/Ror2 in hyperuricemia-induced EMT in kidney tubular injury.Methods
A hyperuricemia model was performed in male Swiss background mice (3 months old, 3040 g) with daily intraperitoneal injections of 125 mg/kg body weight (BW) of uric acid. The mice were terminated on day 7 (UA7, n=5) and on day 14 (UA14, n=5). Allopurinol groups (UAl7 and UAl14, each n=5) were added with oral 50 mg/kg BW of allopurinol treatment. The serum uric acid level was quantified, and tubular injury was assessed based on PAS staining. Reverse transcriptase-PCR was done to quantify Wnt5a, Ror2, E-cadherin, and vimentin expressions. IHC staining was done for E-cadherin and collagen I. We used the ShapiroWilk for normality testing and one-way ANOVA for variance analysis with a PResults
The hyperuricemia groups had a higher uric acid level, which was associated with a higher tubular injury score. Meanwhile, the allopurinol groups had a significantly lower uric acid level and tubular injury than the uric acid groups. Reverse transcriptase-PCR revealed downregulation of the E-cadherin expression. While vimentin and collagen I expression are upregulated, which was associated with a higher Wnt5a expression. However, the allopurinol groups had reverse results. Immunostaining revealed a reduction in E-cadherin staining in the epithelial cells and collagen I positive staining in the epithelial cells and the interstitial areas.Conclusion
Hyperuricemia induced tubular injury, which might have been mediated by EMT through the activation of Wnt5a.Keywords:
Language:
English
Published:
Iranian Journal of Medical Sciences, Volume:43 Issue: 2, Mar 2018
Pages:
164 to 173
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