Detection of Mycoplasma Synoviae Infections in Vaccinated Broiler Breeder Flocks

Mycoplasma Synoviae is a major poultry pathogen which causes significant economic losses to the poultry industry. The aim of study was detection Mycoplasma synoviae infections in vaccinated broiler breeder flocks and their progeny. For this purpose, from 20 broiler breeder farms and their progeny 2 times in 40 and 60 weeks of age 20 tracheal swab and 20 blood samples from each broiler farm were collected. All 20 broiler breeder farms before vaccination against Myc.oplasma Synoviae were confirmed free from Mycoplasma synoviae infections. Tracheal swab transfered to cap tubes containing PPLO broth media and kept at 4°C less than 24 hrs send to laboratory for further proceeding. Antibody against Mycoplasma Synoviae was measured by rapid serum agglutination (RSA) and Elias tests. Tracheal swabs were examined by high resolution melting PCR(HRM PCR) for detection Mycoplasma Synoviae field isolate.. The results showed that vaccination broiler farm against Mycoplasma Synoviae upto 40 weeks could protect all broiler breeder farms from Mycoplasma Synoviae field infection and produce good antibody titers. With increasing age and at age 60 weeks one farms and its progeny was positive for Mycoplasma Synoviae which was differ from Mycoplasma Synoviae included in live vaccine. This study showed vaccination by present vaccine against Mycoplasma Synoviae in broiler breeder farms could not protect older broiler breeder farms in older ages and probably Mycoplasma Synoviae infection transferred to progeny. Therefore vaccinated farms should be monitor by molecular methods.