Study of antioxidant enzymes and lipid peroxidation changes in common carp exposued to silver nanoparticles synthesized using Sargassum seaweed

In this study the activity of antioxidant enzymes and lipid peroxidation in common carp during exposure to silver nanoparticles synthesized using Sargassum angustifolium were investigated. In order to this purpose, after determining the LC50 of silver nanoparticles during 96 h in common carp, fish were exposed to silver nanoparticles concentrations of %0LC50 (control), %1LC50, %10LC50 and 50%LC50 for 14 days. Fish were sampled at 1, 3, 7 and 14 days after exposure. At each sampling liver tissue were isolated and homogenized and then, the activity of catalase (CAT), superoxide dismutase (SOD) and glutathione (GSH), total antioxidant capacity (TAC) and malondialdehyde (MDA) were determined by biochemical methods. The results showed that levels of catalase activity in exposure to high concentrations of silver nanoparticles significantly decreased, but the activity of glutathione and superoxide dismutase increased. According to the The two-way ANOVA and Tukey test, there were no statistically significant differences between the average level of total antioxidant capacity both in liver and gill tissue of different treatments to control and also different days of sampling in each treatment (p>0.05). Malondialdehyde in liver tissue showed a significant increase in 10% LC50 treatment compare with control group (p