Isolation and Specificity of Salmonella enteritidis Bacteriophage from Hospital Sewage Sample

Nowadays, due to the emergence of drug resistance to antibiotics, a good alternative to drugs should be found. Bacteriophages are viruses that play a very important role in the destruction of bacteria. Because of their specific properties, bacteriophages can be considered as suitable substitutes for antibiotics in the treatment of infectious diseases. The aim of this study was to isolate Salmonella enteritidis bacteriophage from hospital wastewater.
Ten samples of sewage, each containing about 100 ml of raw wastewater from Imam Khomeini Hospital of Tehran, were collected and then mixed with culture medium Brain-heart infusion medium (BHI) as a liquid medium for growth of microorganisms. Other stages of the work included purification, bacterial inoculation and determination of host for isolation of bacteriophages. To select the effect of bacteriophage on microorganisms such as: E. coli (ATCC25922), Enterococcus faecalis, Staphylococcus aureus (ATCC2392), Yersinia enterocolitica (ATCC9610), Pseudomonas aeruginosa (ATCC 27853) were used.
Out of 10 samples of bacteriophage sewage selected from Salmonella enteritidis. During the host testing, it became clear that these bacteriophages are well able to leach and destroy Salmonella enteritidis, but have no effect on other bacteria.
The results of this study indicate that the extracted bacteriophage is specifically acting, due to the increase in antibiotic resistance; phages can be used as a suitable substitute for the treatment of Salmonella infections.