Simultaneous spectrophotometric determination of lidocaine and hydrocortisone acetate in pharmaceutical preparations by multivariate calibration methods

The multivariate calibration method, partial least square regression (PLS) was applied for the simultaneous spectrophotometric determination of Lidocaine (LID) and Hydrocortisone acetate (HCA) in their mixtures. The parameters of chemometric technique were optimized and the proposed method was validated with synthetic samples and applied to analyze these drugs in pharmaceutical products with good accuracy and precision. The results were compared with the HPLC method. The squares of correlation coefficients (R2) for predicted LID and HCA with the proposed method in test samples were 0.9970 and 0.9964, respectively. The relative standard deviations for commercial products were less than 1%.