Comparison of the effects of aerobic exercise and low-carb diet on Vaspin and Interlukine-6 in type II diabetic rats

Changing the life style is the most successful way for prevention and treatment of type II diabetes and negative energy balance is one of its methods. The aim of this study was to evaluate the interactive effects of calorie restriction alone and in combination with aerobic exercise on Vaspin and Interlukine-6 (IL-6) in type II diabetic male rats. In an experimental study, 56 male Wistar rats (age: 10 weeks) were randomly classified into seven groups including one healthy control and six diabetic groups as control, aerobic exercise, standard diet, low carb diet, standard diet plus aerobic exercise, and low-carb diet plus aerobic exercise. Twenty percent of negative energy balance was carried out on a daily basis on all groups for eight weeks. Blood samples were collected at the end of protocol and adipokines were analyzed by ELISA method. The results showed that Vaspin levels in diabetic control group were significantly decreased in contrast to healthy control group (p≤0.05). Aerobic exercise in combination with low-carb diet and standard diet also caused a significant increase in the Vaspin level compared to diabetic control group. Also, the results showed that induction of diabetes significantly increased the level of IL-6 compared to healthy control group. However, there was no significant difference in the level of IL-6 between the experimental groups and diabetic control group. This study showed that combination of aerobic exercise with low-carb diet and standard diet can increase pro-Inflammatory adipokines (Vaspin) but does not significantly affect anti-Inflammatory adipokines (IL-6) level