Increased hydrogen peroxide and antioxidant enzymes activity in Iranian poppy cells treated with methyl jasmonate and salicylic acid

Salicylic acid and Jasmonates are the natural occurring hormones which involved are in defense mechanisms of plant against biotic and abiotic stresses. In this research, the effect of methyl jasmonate and salicylic acid on content of hydrogen peroxide, proline and total protein and antioxidant enzymes activity were investigated. Cell suspension culture of Iranian poppy was established on liquid MS medium containing 2,4-D, kinetin and ascorbic acid. The cells treated with methyl jasmonate (100 µM) and salicylic acid (75 mg/l), and the cells were evaluated in times 12, 24, 36, 48 and 144 hours after treatment. The results indicated that medium pH increased after cells treatment with methyl jasmonate and salicylic acid. The cell growth and viability were decreased by elicitor treatments, compared to control cells and this reduction was higher on 6 days after treatment. Amount of hydrogen peroxide in the cells treated with methyl jasmonate at all assessed periods was significantly higher than those of cells treated with salicylic acid and control cells. Proline and total protein content and antioxidant enzymes activity of cells were increased significantly by methyl jasmonate and salicylic acid as compared with control cells. The highest activity of Catalase in 48 hours after treatment with salicylic acid and the highest activity of superoxide dismutase in 144 hours after treatment of cells with methyl jasmonate was obtained.