The Effect of Metyl Jasmonate Hormone on Gene Expression of Phenylalanine Ammonia Lyase in Arctium lappa L

performed using specific primers designed from conserved sequences of the gene in NCBI database. The sequence analysis (609 bp) revealed that the detected PAL gene is similar to those previously sequenced in the NCBI database. Methyl jasmonate is a cyklopentany compound of linolenic acid derivatives which can be produced through the octadecanoid pathway. There are evidences that methyl jasmonate as a signaling molecule interference in some signaling pathways that induces certain enzymes catalyze biosynthetic reactions for the formation of the defensive reaction and leading to the induction of immune responses. In the current study, gene expression of PAL gene was evaluated by applying of 50 and 100μm MeJA, at intervals of 24, 48 and 72 hours. Results showed that expression of PAL gene significantly increased in response to MeJA after 24h. The gene expression of PAL was significantly higher after 24h treating samples with 100μm MeJA compared to the control. The results of this study suggest that metyl jasmonate is an efficient abiotic elicitor which affect expression of PAL invovled in polyphenol compounds in Burdock