Association Analysis for Morphological Traits in Iranian Basil Accessions Using ISSR Marker

The aim of the present study was to map the morphological traits in Iranian Basil accessions (Oscillum Oscillos) by Inter Simple Sequence Repeats (ISSRs) markers.  In this experimental study, 50 Iranian basil accessions from different geographical regions were used and the experiment was based on a completely randomized design. Extracting DNA and PCR was performed with 12 ISSR primers for Basil accessions. Components of variance, general heritability, and genetic and phenotypic variation coefficients were calculated by formula. Bayesian method, linear mixed model as well as Minitab 17, DARwin 5, Structure 2.3.3, Tassel 3, and SPSS 20 software were used.  There was a positive correlation between the majority of traits for basil accessions. The highest coefficient of genetic variation was observed in stem diameter and internode length and the lowest was observed in flower number. Heritability varied between 3.63% and 94.24%. Foutrteen loci with 7 traits were recognized. The range of phenotypic changes varied from 3% to 29%. The highest number of locus was obtained for stem diameter traits and the lowest was obtained for lateral branch number. Six loci were specifically associated with only one trait and other loci were common in traits. The phenotypic variation varied between 17% and 29%.  Traits have a wide variety in Basil accessions and there is a positive correlation between the majority of them. The heritability of the traits varies from 3.63% to 94.24% and the range of the phenotypic changes varies from 3% to 29%. The highest number is for stem diameter traits and lowest is for lateral branch number. Six loci are specifically associated with only one trait and other loci are common in traits. The phenotypic variation varies between 29% ‐17%.