Investigation of Serum Level and IL-13 Genetic Variations in Positions +2044 G/A and -1512A/C in Southern Iranian Patients with H. Pylori

Helicobacter pylori (H. pylori)is agramnegative bacterium thatcolonizes stomach mucosa andcausesupper gastrointestinal diseases.Interleukin-13 is a preventive immune cytokine produced by many immune cells. IL-13 gene is located on chromosome 5 q 25 bp. It has polymorphisms at positions -1512 A/ C and +2044 G/A, which are associated with the expression rate of this cytokine .The aim of this study is to investigate the serum level and polymorphisms of IL-13 gene in patients with H.pylori infection. In this case control study, DNA was isolated by salting out method from peripheral blood of 150 patients with H.pylori infection diagnosed by an expert. PCR-RFLP was used to examine polymorphisms of IL-13 gene . The results were compared with 150 healthy controls by SPSS software and Chi-square test.Tostudy, the serum levels of IL-13 cytokine, serum samples of 40 patients and 40 controls were prepared and examined using ELISA kit. According to the findings, there is a significant difference in (CI: 06/1-09/0, OR 32.0 MB) GA (P = 0/005) and (08/1-52/0: CI, OR: 45.0) GG (P = 0/045) genotypes at A/2044 G +, position between patients and the control group. No significant differences were observed at the position of -1512 A/C. Also, no significant differences were seen between serum level of IL-13 cytokine and H. pylori disease (P >. 0.05). Based on the results obtained, it seems that GA and GG genotypes of interleukin-13 gene at position 2044+ make people more susceptible to H. pylori infection.