Comparison of Human Mesenchymal Stem Cells Derived from Various Compartments of Human Adipose Tissue and Tunica Adventitia Layer of the Arteries Subsequent to Organ Donation
Author(s):
Article Type:
Research/Original Article (دارای رتبه معتبر)
Abstract:
Background
Mesenchymal stem cells are one of the most interesting cell sources used in regenerative medicine.
Objective
In the present study, we isolated and characterized the mesenchymal stem cells from various compartments of human adipose tissue and tunica adventitia layer of the arteries.
Methods
Tissue explant culture was done from various compartments of the human adipose tissue and tunica adventitia layer of the arteries, including adipose tissue far from the vessels, perivascular tissues that are completely attached to the vessels, and tunica adventitia layer of the arteries. After the cell culture, characterization of the cells was determined at 3rd–5th passages. Flow cytometry was performed for antigen expression analysis of CD34, CD45, CD44, CD90, CD29, CD73, and CD105. For the evaluation of cell differentiation potential, adipogenic and osteogenic differentiation was conducted under appropriate protocols.
Results
The cells were positive for CD44, CD90, CD29, and CD73 and negative for CD34, CD45, and CD105. Adipogenic and osteogenic differentiation potentials were different among the cells from various compartments. The cells derived from perivascular tissue demonstrated better adipogenic and osteogenic differentiation.
Conclusion
It is essential to characterize the cells from different tissues and compartments for different purposes in regenerative medicine.Keywords:
Language:
English
Published:
International Journal of Organ Transplantation Medicine, Volume:10 Issue: 2, Spring 2019
Pages:
65 to 73
magiran.com/p1991810
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