Effect of different sinusoidal-vibration frequencies on some physiological parameters, antioxidant activity and total phenol of Anthemis gilanica Bornm & Gauba

Mechanical vibration is one of abiotic stresses which could affect on growth and development of plant cells. In this research, effect of sinusoidal vibration were studied on some physiological parameters, protein, total phenols and free radical scavenging activity using 2,2-diphenyl-1-picrylhydrazyl (DPPH) in Anthemis gilanica seedlings. Sinusoidal vibrations were applied at 0, 10, 15, 50 and 100 Hz on 10 days seedlings, and were analyzed for physiological and biochemical responses after 30 days. Results showed different responses of growth, antioxidant activity and phenolic content under different frequencies of vibration. Sinusoidal vibrations at frequencies of 15 and 50 Hz resulted in a significant increase in growth parameters, relative water content, chlorophyll a and chlorophyll b and protein content compared to control. Malondialdehyde (MDA) and H2O2 levels decreased significantly at frequencies higher than 10 Hz, and 15 Hz treatment resulted in a 54.55% and 24.5% reduction of hydrogen peroxide and malondialdehyde content as compared to control. Sinusoidal vibrations also increased in free radical scavenging activity by reducing the IC50 value, and the highest decrease was observed at 50 Hz frequency. It seems that increase of antioxidant capacity under proper frequency of vibration can induce A. gilanica growth.