Green synthesis and antibacterial activity of zinc selenide (ZnSe) nanoparticles
Objective(s)Drug delivery is an engineering technology to control the release and delivery of therapeutic agents to target organs, tissues, and cells. Metallic nanoparticles, such as gold nanoparticles (AuNPs) have exceptional properties which enable efficient drug transport into different cell types with reduced side effects and cytotoxicity to other tissues.
Materials and MethodsAuNPs were synthesized by adopting the Turkevich method to reduce tetra chloroauric (III) acid (HAuCl4) solution with sodium citrate. A factorial design of 24 was used to investigate the influence of temperature, stirring speed, and the volume of citrate and gold salt on the size of AuNPs synthesis. The produced chemical-AuNPs (CN-AuNPs) were characterized using ultraviolet-visible spectroscopy and dynamic light scattering (DLS) which was conjugated with polyethylene glycol (PEG) loaded with chloroquine diphosphate. The latter were characterized with transmission electron microscopy (TEM), Energy dispersive x-ray spectroscopy (EDS), selected area electron diffraction (SAED) patterns and Fourier transmission infrared spectroscopy. The antimalarial activities of the three formulations were tested on Plasmodium-infected mice. Moreover, the evaluation of curative potentials of the formulations was carried out via parasite counts. The anemic and pathological conditions of nano-encapsulation were investigated for their cytotoxicity level.
ResultsThe CN-AuNPs show surface plasmon resonance absorption ranging from 526 to 529 nm with smaller particle size at the lower citrate volume. The TEM image of CN-AuNPs with polyethylene glycol (PEG) and CN-AuNPs-PEG encapsulated with chloroquine diphosphate revealed spherical shape with EDS showing the appearance of gold (Au) at 2.0, 2.1, and 9.9 KeV. The SAED also revealed that the AuNPs were crystalline in nature. The in vitro time-dependent encapsulation release showed an extension of time release, compared to CN-AuNPs-PEG with parasitemia clearance at the same level of cytotoxicity.
ConclusionTherefore, although improved activity of the CN-AuNPs-PEG encapsulating was achieved but its cytotoxicity still is a limitation.
Nanomedicine Journal, Volume:6 Issue:4, 2019
258 - 262
روشهای دسترسی به متن این مطلب
در سایت عضو شوید و هزینه اشتراک یکساله سایت به مبلغ 300,000ريال را پرداخت کنید. همزمان با برقراری دوره اشتراک بسته دانلود 100 مطلب نیز برای شما فعال خواهد شد!
به کتابخانه دانشگاه یا محل کار خود پیشنهاد کنید تا اشتراک سازمانی این پایگاه را برای دسترسی همه کاربران به متن مطالب خریداری نمایند!