Evaluation of the effect of salinity stress induced by sodium chloride on morphological, physiological and biochemical traits and proteome pattern of autumn rapeseed

In order to investigate the response mechanism to salinity stress in autumn rapeseed, Orient tolerant cultivar was evaluated in greenhouse condition and salinity stress began at the stem elongation stage and two weeks after stress, leaf samples were prepared. The results showed that salinity stress reduced the value of morphological and physiological traits, caused imbalance in ionic concentrations by increasing the concentration of sodium ion and decreasing intracellular potassium ion, as well as decreasing potassium/sodium ratio within leaf cells. Meanwhile, under salinity stress, the relative water content of the leaves decreased, but the concentration of proline and glycinebetaine was higher than that of control condition. Also, the results of the proteome analysis showed that among the control and treatment of severe salt stress in this cultivar, among 118 protein spots, 8 repeatable protein spots had a significant difference and also a significant induction factor. The function of the identified proteins in the studied cultivar were related to cell homeostasis, energy and carbohydrate metabolism, photo-reaction of photosynthesis, and transcription regulation (protein synthesis).