Genotyping of JC virus in clinical samples in North Eastern Iran

Determination of genotype of JC virus in northeastern Iran to finding any different genotype in different tissues. Background Data is lacking about genotypes in Iran and Due to the association of some genotypes of JC virus with PML (Progressive multifocal leukoencephalopathy) And Due to the fact that some genotypes are more likely to be associated with certain diseases, such as cancer, the importance of this research is highlighted.

 Twenty two colorectal tissue samples collected from Colorectal cancer patients and 19 urine samples collected from healthy individuals and after genomic extraction, PCR was applied to amplify the desired gene region for genotyping. Sequences reported with sequences recorded in the NCBI database were compared. The 100% match was observed in most samples.

Conclusion According to statistical analysis, there is no correlation between urinary tract genotypes and tissue samples. Although genotype 2 was observed only in urine specimens and was not observed in tissue samples. The most common genotype in this study was Type-1 and then Type-3. We did not see other genotypes in this study.