The Study of Activity of Antioxidant Enzymes, Guaiacol Peroxidase and Ascorbate Peroxidase and the amount of Na, K and pigment content in Spinach oleracea L under NaCl Salinity Stress

Salinity is one of the most important abiotic stresses which with produce a reactive oxygen species affect on production and quality of herbal products. Plants are using antioxidant systems such as ascorbate peroxidase and guaiacol peroxidase to deal with different types of free radicals.Also, salinity stress, affect on chlorophyll content and causes ions to disrupt the homeostasis of sodium, potassium, chloride, and calcium. For this purpose, these parameters in spinach in resistance to salt stress were studied. In this study, Salinity at four levels( 0,200,400 and 600 Mm) in a period of 14 days after the stress and intermittent interval (72 hours)in a factorial and completely randomized experiments with the model of base design were considered. Ascorbate peroxidase and guaiacol peroxidase activities were measured using standard method. Also, Ions of sodium, potassium and chlorophyll and carotenoid content in the treatment of salinity stress were measured. Data obtained from this study using the statistical software SAS and MSTATC were analyzed. Our results indicated that the effect of salinity stress on the activity of anti-antioxidant enzymes, ascorbate peroxidase, and guaiacol peroxidase was significant. The results showed that with increasing salinity level, the sodium and potassium ions in leaf compare to control has been significantly increased and reduced, respectively. The concentration of chlorophyll and carotenoid pigments decreased with increasing salinity level.