C-, G-, NOR- and restriction enzyme- banding and DAPI staining of chromosomes of Zagros pupfish Aphanius vladykovi

Zagros pupfish( Aphanius vladykovi) is a native species of Iran living in Zagros mountains (in Chaharmahal- va- Bakhtiari province). Because of the close habitats, this species is important in terms of biodiversity and evolution studies. several methods of staining and banding protocols including DAPI staining as well as C-, G-, NOR- and restriction enzyme- banding were used in this study. Diploid chromosome number for this species was found with modal number of 2n= 48. G- banding produced distinguishable bands, however, C-banding did not show any clear bands suggesting lack of protein rich regions. Ag – NOR banding resulted in several bands particularly in telomeric regions of the chromosomes. In RE- banding experiments Alu I and BamH I restriction enzymes produced clear bands whereas Pst I failed to produce any clear bands